Evaluation of two commercial real - time PCR assays for the detection of 1 Mycoplasma genitalium in urogenital
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Evaluation of two commercial real-time PCR assays for detection of Mycoplasma genitalium in urogenital specimens.
The performance of two commercial real-time PCR kits for the detection of Mycoplasma genitalium was evaluated in comparison to an in-house real-time PCR assay. Concordances of 96% and 93% were found for the TIB MOLBIOL and the Diagenode assays, respectively, compared to the results of the in-house assay.
متن کاملDevelopment of TaqMan Duplex Real-time PCR for Simultaneous Detection of Chlamydia Trachomatis and Mycoplasma Genitalium
Background and Objective: Sexually infections transmitted by bacteria are one of thetherapeutic and social problemsworldwide. The Real-time PCR assay is one of the most sensitive diagnostic and screening methods for these infections. The purpose of this study wassimultaneous detection of Chlamydia trachomatis and Mycoplasma genitaliumusing the TaqMan duplex real-time polymerase chain reaction. ...
متن کاملEvaluation of a Commercial Multiplex Quantitative PCR (qPCR) Assay for Simultaneous Detection of Mycoplasma genitalium and Macrolide Resistance-Associated Mutations in Clinical Specimens
Macrolide antibiotics are the first-line treatment for Mycoplasma genitalium infections; however, macrolide resistance has increased up to 40% in several countries (1–3). Consequently, the 2016 European guideline on M. genitalium infections has recommended complementing the molecular detection of M. genitalium with an assay capable of detecting macrolide resistance-associated mutations (4). We ...
متن کاملEvaluation of five real - time PCR assays for detection of Mycoplasma pneumoniae 7 8 Running title : Real - time PCR for detection of
25 Four commercial real-time PCR assays to detect M. pneumoniae were tested and the results 26 compared with an in-house approach. Despite differences of crossing threshold values up to 4, 27 assays were able to detect at least 20 cfu/5 μl (52 fg DNA/5 μl) of sample with best clinical 28 sensitivity of Diagenode kit. 29
متن کاملA comparative study of three different PCR assays for detection of Mycoplasma genitalium in urogenital specimens from men and women.
The aim of this study was to compare conventional 16S rRNA gene PCR, real-time 16S rRNA gene PCR and real-time Mycoplasma genitalium adhesin protein (MgPa) gene PCR as detection methods for M. genitalium infection. The study also determined the prevalence of M. genitalium in male and female patients attending a sexually transmitted infections clinic in a rural area in the west of Sweden. First ...
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